1. Isolate DNA from Hair Shaft
2. Set up PCR Reaction
a. Add Reagents to PCR Tubes

1. Taq Polymerase
2. Tris - HCl
3. KCl
4. MgCl
5. dNTP
b. Add Mt DNA Primer and Loading Dye Mix to all reaction tubes
1. mt primers
2. sucrose
3. cresol red in tris-low EDTA 
4. buffer
3. Program the thermal cycler for 30 cycles and start thermal cycler

94 Degrees C - 30 seconds
55 Degrees C - 30 seconds
72 Degrees C - 30 seconds

4. Load the Standard and PCR Products
5. Electrophorese the Standard and PCR Products
6. Stain the Gels with Carolina Blu
7. View Gels
8. Observe Results