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Laboratory Report Outline: RFLP

M. Villani

Introduction:
1. What is meant by RFLP?
2. When are RFLP tests conducted in forensic science labs?
3. What can be learned about a suspect from examining RFLP results?

Problem: Who killed the woman found at the crime scene?

Hypothesis: 
Based on the information given by the police (information given in the introduction of your lab sheets), who do you think killed the woman found at the crime scene?

Data:
1. Make a drawing of what your table's gel looked like under electrophoresis.
2. Write a paragraph to explain your results to the members of a jury.
3. Compare your results with the actual results.
4. Answers the questions related to the following tapes:
a. electrophoresis
b. southern blotting
c. hybridization

Analysis:
1. Compare the protocol used in class with the protocol used in a forensic
DNA lab in terms of:
a. equipment needed 
b. reagents required 
c. purpose for each piece of equipment and each reagent used during the procedure.
2. Compare the results obtained by your group with the actual results.

Conclusion:
1.Why are DNA bands important pieces of forensic evidence?
a. How do the bands in each of the four lanes compare?
b. Where can the larger molecules be found? Why?
c. Where can the smaller molecules be found? Why?
2. What are some sources of error in this type of experiment?
3.Why must the digest be incubated at 37 degrees C for 20 or more 
minutes?
4.Why is the loading dye added to each restriction reaction?
5.Why is the gel subjected to electrophoresis?
6. Why must the gel be stained?
7. Why must the gel be destained?
8. What are two ways of staining the gel?
9. Why must the gel be destained?
10. Why did we use methylene blue rather than ethidium bromide in our 
study?
11. What are the advantages of staining the gels with ethidium bromide?
12. What restriction enzymes did we use in our study?
a. How does each of these restriction enzymes work?


Copyright Bronx Science 2001